Clonogenic capacity
WebSince several programs throughout the world are currently evaluating the feasibility of large-scale UCB banking for unrelated transplants, it was the aim of this study to evaluate … WebSince several programs throughout the world are currently evaluating the feasibility of large-scale UCB banking for unrelated transplants, it was the aim of this study to evaluate whether cypropreservation procedures might heavily impair the clonogenic capacity, the feasibility of CD34+ selection and the ex vivo expansion potential of UCB ...
Clonogenic capacity
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WebThe clonogenic assay is an in vitro cell survival assay that evaluates all modalities of cell death based on the ability of a single cell to grow into a colony. The colony is defined to … WebRPRML overexpression significantly inhibited clonogenic capacity, anchorage-independent growth, and proliferation in vitro. Circulating methylated RPRML DNA distinguished patients with gastric cancer from controls with an area under the curve of 0.726. The in vitro overexpression results and the poor patient survival associated with lower RPRML ...
WebDec 21, 2015 · Since a clonogenic capacity assay in a semi-solid media remains the gold standard technique to study the LSC function ex vivo, primary AML samples were treated with G-CSF for 18 h and a significant reduction of CFU-B colonies was observed in a dose-dependent manner upon treatment with G-CSF (Fig. 4a), suggesting that G-CSF exerts … WebApr 8, 2024 · Clonogenic survival assay Single-cell suspensions were inoculated into six-well plates at densities of 400–12,000 cells per well. After 12 h, cells were irradiated with six MV X-rays at gradient doses of 0, 2, 4, 6, and 8 Gy using an X-ray linear accelerator (Clinac 2100EX, Varian Medical Systems) with a 300 cGy/min dose rate and 100 cm ...
WebDec 7, 2024 · Culture of CD34 + cells with HIF-MSC increases their clonogenic capacity in vitro. Regarding the evaluation of in vitro functional effects induced after co-culture, we observed that the capacity of CD34 + cells to form CFU-GM colonies was significantly higher when they were co-cultured with HIF-MSC in comparison with MSC WT (p = 0.048 WebFeb 28, 2013 · The clonogenic capacity of CD44 + /CD133 low and CD44 + /CD133 high cells was determined. The results were obtained by microscopic observation; bars …
WebMay 16, 2024 · The remarkable tumor suppressor capacity of PCNP in vivo was confirmed by IHC analysis for proliferation (Ki67), associated angiogenesis (CD31), apoptosis (cleaved caspase-3), cell cycle (p21), autophagy (Beclin-1) of tumor sections from tumors harvested from control, Mock, PCNP, sh-sub, and sh-PCNP group mice. PCNP may act as a …
WebThe proliferative capacity, assessed as population doubling time (PDT), demonstrated no difference for MSC from BM pos and BM neg samples indicating that the proliferation of the total MSC population does not only depend on the … boston north end historyWebJul 22, 2009 · We have previously shown that reduced Erk phosphorylation as a consequence of the U0126 inhibitor, correlates with reduced clonogenic and proliferative capacity of K562 cells. CCN3 may compromise cell survival by reducing Erk phosphorylation causing decreased expression of pro-survival factors such as BCL-2, … boston north end food tourWebAug 22, 2024 · It showed that tanshinone IIA inhibited radiation-induced cardiomyocyte injury while enhancing the proliferative capacity of the cells. When further administration of p38 MAPK agonist treatment, it was found that the cell clonogenic rate decreased significantly ( ), and the LDH level also increased significantly ( ), suggesting that p38 … boston north end foodWebFeb 18, 2024 · Treatment with rhTPO both at one month and two months post-TBI showed stronger clonogenic capacity of HPCs and competitive hematopoietic reconstitution after transplantation, and corrected the myeloid cell skewing seen in the cells from the irradiated mice treated with vehicle after transplantation. This suggested that rhTPO may … hawkmoon feather 2WebFurther, clonogenic assays which is widely accepted tool in tumor biology to measure tumor-initiating capacity and stem cell status was used to check the ability of Ketorolac to inhibit size, and number of colonies formed by cells alone and in combination with Sunitinib. Our data indicates strongly abolished clonogenic capacity of RCC cells. hawkmoon feather locationsWebOct 25, 2024 · The clonogenic assay measures the capacity of single cells to form colonies in vitro. It is widely used to identify and quantify self-renewing mammalian cells … boston north end lodgingWebFeb 1, 2006 · clonogenic capacity and, as a consequence, larger numbers of. tumor cells are requir ed to yield tumors in recipient animals. From. a few transplantable tumors in rats and mice, harvested cells ... boston north end hotels