WebDQ-ovalbumin (OVA) is a phagolysosome-localized fluorescent dye with a localized small vesicle pattern in untreated macrophages ( 5 ). As expected, challenge with silica induced the conversion of DQ-OVA fluorescence from a small vesicle pattern to a spread cytosolic pattern in Mcufl/fl BMMs, indicating the leak of phagolysosomes ( Fig. 2 A ). Web26 ago 2024 · Wnt5A mediated DQ-OVA processing is associated with the proteasome regulator PA28α-PA28β: Confocal microscopy (A, B) along with Mander’s overlap coefficient analysis (C, D) n = 2, 6-14 fields, each dot representing a field and ImageJ intensity measurement (E, F) n = 3, 7-8 fields, each dot representing a cell, demonstrate a …
JCI - Antigen-loaded monocyte administration induces potent …
Web17 apr 2015 · A, Histograms of mean fluorescence intensity (MFI) of DQ-OVA uptake and processing in a representative patient from each children group (CD, UC, and HC). B, Overall DQ-OVA stainings in DCs pretreated with medium alone or Tribif. Results are shown as MFI of DQ-OVA expression obtained in 9 CD, 6 UC, and 6 HC pediatric patients. Web11 mag 2015 · DQ-OVA is minimally fluorescent while OVA is intact, but upon proteolytic processing of OVA, the fluorophore is unquenched, resulting in a large increase in fluorescence. Cell culture media on the cells was replaced with fresh TC-1 media, and the NE-OVA solution was then added to the media to obtain final concentrations of 0.025%, … boom on a boat definition
Development of endocytosis, degradative activity, and …
Web10 mag 2024 · Degradation of DQ-OVA (increasing fluorescence) was measured using flow cytometry. To control for differences in uptake capacity, sorted cell populations were … WebMice were intranasally immunized with hPGp ± R848 (A-C) or DQ-OVA ± R848 (D) and 24hpi cellular composition and uptake of DQ-OVA in the cervical lymph node was analyzed. Web13 gen 2024 · Assessing the Antigen Processing Capacity of Cells Using DQ-Ovalbumin (DQ-OVA) DQ-OVA is a self-quenched conjugate of ovalbumin which is strongly labeled … boom of tower crane