WebMar 6, 2024 · Interestingly, the single-allele topology clusters are cell type-specific even inside highly conserved TADs in different types of cells. In summary, HiPore-C enables … We developed a protocol of in situ high throughput multi-way contact long read Pore-C sequencing (in situ HiPore-C), a strategy that integrated multi-fragment ligates preparation with third-generation sequencing technology. Compared to the reported Pore-C method, HiPore-C can yield more chromatin … See more This pipeline has a number of dependencies including the following: 1. python (3.7); 2. Guppy (4.5.3); 3. Megalodon (2.3.4); 4. Nanoplot (1.30.1); 5. seqkit (2.2.0); 6. … See more In this step, to obtain more accurate alignment results for high-order reads, and to improve the effective usage of HiPore-C data, we introduced the NGMLR and Minimap2 to construct the HiPore-C alignment pipeline. … See more In this step, Guppy and Megalodon software were used. And due to the large amount of Nanopore data, it is recommended to generate a corresponding fastq or modification callling bam result for each … See more To compare with the Hi-C data and the subsequent visualization, the multiway contacts of HiPore-C reads were decomposed into pairwise contacts using Generate_Contact_juiceMatrix.py, … See more
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Webservlet介绍基本的架构就是客户端和服务器; 客户端通过网络向服务器发送请求, 服务器向客户端返回相应; 在服务器中, 服务器会启动一个服务, 来接收客户端的请求, 并且搭载对应 … WebIn summary, HiPore-C enables global characterization of single-allele topologies at an unprecedented depth to reveal elusive genome folding principles. WebMay 2, 2024 · Dilichukws task 0. a1cc558 on May 2, 2024. 189 commits. 0x00-hello_world. task 6. last year. 0x01-variables_if_else_while. task 12. last year. para powerlifting training in birmingham