WebIn the absence of activation, the constitutive HIS3 expression from PminHIS3 is very low. During library screening, the leaky expression of HIS3 is controlled by adding 3-amino-1,2,4-triazole (3-AT) to the medium. The concentration of 3-AT needed to fully suppress leaky HIS3 expression must be determined empirically for each DNA target element ... WebJul 11, 2016 · PminHis3 and P CYC1 represent two minimal promoters from the His3 gene and yeast cyclin gene, respectively. P GAP indicates the promoter of the glyceraldehyde 3 …
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WebInternational Journal of Molecular Sciences Article ThHSFA1 Confers Salt Stress Tolerance through Modulation of Reactive Oxygen Species Scavenging by WebOct 5, 2013 · 为了消除最基本启动子PminHIS3 和PCYC1 的本底, 在培养基中加入10 mmolPL 3AT, 就可以很好地抑制 假阳性克隆的生长。 cDNA AD 融合表达载体的转 化方法和步 … how to know if vizio tv has smartcast
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WebMPHI is a major contributor to community health in Michigan and our staff is comprised of more than 1000 individuals from a wide range of backgrounds and expertise. Our staff … WebMatchmaker One-Hybrid Library Construction & Screening Kit WebOct 18, 2024 · It contains a HIS3 nutritional reporter gene, located downstream of a multiple cloning site (MCS) and the minimal promoter of the HIS3 locus (PminHIS3). Cis-acting … joseph shearing - the golden violet